Application of mutated Clostridium difficile toxin A for determination of glucosyltransferase-dependent effects.

نویسندگان

  • Matthias Teichert
  • Helma Tatge
  • Janett Schoentaube
  • Ingo Just
  • Ralf Gerhard
چکیده

Mutation of tryptophan-101 in Clostridium difficile toxin A, a 308-kDa glucosyltransferase, resulted in a 50-fold-reduced cytopathic activity in cell culture experiments. The mutant toxin A was characterized and applied to distinguish between glucosyltransferase-dependent and -independent effects with respect to RhoB up-regulation as a cellular stress response.

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عنوان ژورنال:
  • Infection and immunity

دوره 74 10  شماره 

صفحات  -

تاریخ انتشار 2006